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Fluorescent Dye-Labels

Fluorescent dyes are the primary means of labeling biomolecules. Fluorescently labeled reporter molecules can provide biological information at the molecular level in living systems, enable visualization of the specific molecular events in disease process, and monitor therapeutic responses. As an important molecular imaging technology, fluorescent imaging has specific advantages over other imagaing techiques, such as high sensitivity, use of nonradioactive materials, and easy detection by lower cost instruments. HongTide can incorporate fluorescent dyes at variety of positions in a peptide, including the (1) N-terminus, (2) C-terminus, and (3) side-chains of amino acids such as lysine, glutamic acid, and asparatic acid. Here are some popular fluorescent dyes we usually use below and please contact me if your fluorescent dye is not on the list.
FITC (Fluorescein isothiocyanate)

Fluorescent dyes

FITC

Color

Green

Ex(nm)

495nm

Em(nm)

521nm

Labeling position

N-terminal, Lys side amide

FAM

Fluorescent dyes

FAM

Color

Green

Ex(nm)

492nm

Em(nm)

517nm

Labeling position

N-terminal, Lys side amide

Rhodamine B

Fluorescent dyes

Rhodamine B

Color

Light red

Ex(nm)

543nm

Em(nm)

565nm

Labeling position

N-terminal, Lys side amide

TAMRA (5-Carboxytetramethyl rhodamine)

Fluorescent dyes

TAMRA

Color

Orange

Ex(nm)

543nm

Em(nm)

572nm

Labeling position

N-terminal, Lys side amide

Cy5 (1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride)

Fluorescent dyes

Cy5

Color

Red

Ex(nm)

650nm

Em(nm)

670nm

Labeling position

N-terminal, Lys side amide

MCA (7-Methoxycoumarin-4-acetic acid)

Fluorescent dyes

MCA

Color

Purple

Ex(nm)

325nm

Em(nm)

393nm

Labeling position

N-terminal, Lys side amide

AMC (7-Amino-4-methylcoumarin)

Fluorescent dyes

AMC

Color

Blue

Ex(nm)

351nm

Em(nm)

450nm

Labeling position

C-terminal

AFC (7-Amino-4-trifluoromethylcoumarin)

Fluorescent dyes

AFC

Color

Blue

Ex(nm)

382nm

Em(nm)

480nm

Labeling position

C-terminal

Dansyl (5-(Dimethylamino) naphthalene-1-sulfonyl)

Fluorescent dyes

Dansyl

Color

Yellow

Ex(nm)

342nm

Em(nm)

560nm

Labeling position

C-terminal

EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid)

Fluorescent dyes

Dansyl

Color

N/A

Ex(nm)

340nm

Em(nm)

490nm

Labeling position

C-terminal

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