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Introduction of N-terminal acetylation / C-terminal amidation

N-terminal acetylation and / or C-terminal amidation can reduce the overall charge of the peptide, but the solubility may decrease. Because it can increase the stability of the polypeptide, and the end closure makes it closer to the maternal protein, such modification has the potential to enhance the stability of the polypeptide Possibility of biological activity.
Advantages of N-terminal acetylation / C-terminal amidation
  • It eliminates the terminal charge of the polypeptide, makes it closer to the maternal protein, and enhances its ability to enter cells. If your experiment is related to intracellular, in vivo or in vitro function research, we recommend that the synthesized peptide be acetylated at the N-terminal/C-terminal amidation modification.

  • It enhances the metabolic stability of the peptide and improves its resistance to Exopeptidases such as protease, terminal lyase and synthase.

  • Amidation can not only enhance the activity of peptide hormones, but also prolong their shelf life.

  • It can eliminate the influence of amino terminal (n) and / or carboxyl terminal (c) charges on ELISA detection experiment.